A whole-genome scan was conducted using 132 microsatellite markers to identifychromosomal regions that have an effect on teat number. For this purpose, anexperimental cross between Chinese Meishan pigs and five commercial Dutch piglines was used. Linkage analyses were performed using interval mapping byregression under line cross models including a test for imprinting effects. Thewhole-genome scan revealed highly significant evidence for three quantitativetrait loci (QTL) affecting teat number, of which two were imprinted. Paternallyexpressed (i.e., maternally imprinted) QTL were found on chromosomes 2 and 12. AMendelian expressed QTL was found on chromosome 10. The estimated additiveeffects showed that, for the QTL on chromosomes 10 and 12, the Meishan allelehad a positive effect on teat number, but, for the QTL on chromosome 2, theMeishan allele had a negative effect on teat number. This study shows thatimprinting may play an important role in the expression of teat number.