A tool for designing gene-specific primers targeted at genomic regions of interests based on known gene structure and EST sequence information
The Expeditor is a web-based tool to combine human intron/exon structural information for a given gene and EST sequence information of the target species (or a good consensus coding sequence from multiple species), to design primers for amplification of genomic segments of a gene.
This tool has been published on Journal of Heredity, January 2005 issue. Users may cite the paper as:
Hu, Zhi-Liang, Kimberly Glenn, Antonio M. Ramos, Charles J. Otieno, James M. Reecy and Max F. Rothschild (2005) Expeditor: A Pipeline for Designing Primers Using Human Gene Structure and Livestock Animal EST Information. Journal of Heredity, Volume 96, Number 1 (January 2005), pp. 80-82
This software is free for use by not-for-profit or academic users. However the users are strongly encouraged to fill out a survey form. Feedbacks for improvement of the software is most desirable. Please use our Survey/Feedback form.
The Expeditor is made intuitively easy to use (See our Three-Step-Guide). One can simply follow instructions at each step as you go through the designing process on this web tool. For further user support and feedbacks, please fill our Survey/Feedback form.
THIRD PARTY SOFTWARE:
Some 3rd party software / rules are embeded in Expeditor. We want to acknowldge the following:
This work is supported by funds from the USDA National Animal Genome Research Program, Pig Genome Coordination and Bioinformatics Coordination Programs.
© 2003-2024 USA - USDA - NRSP8 Program for Applied Bioinformatics.
Contact: Bioinformatics Team