| . | Forward Primer Sequencesa | Reverse Primer Sequencesa
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| EST | Clone Length (kb) | Database Accession Number | Length EST Seq (bp) | Match Number/ Description | Match Score | Database Accession Number | Length EST Seq (bp) | Match Number/ Descriptionb | Match Score
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| M10 | 0.9 | AA063647 | 443 | (n) X62880 muscle CRC (pig) | 1100 | . | . | . | . | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| M13 | 2.1 | L20456 | 387 | No matches | . | AA063648 | 239 | No matches | . | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| M29 | 1.0 | L20460 | 322 | (n) X65036c (p) X65036 muscle a7-integrin (r) | 441 188 | AA063649 | 332 | (n) X65036 muscle a7-integrin (r) | 643 240 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| M63 | 1.5 | L20461 | 341 | No Matches | . | AA063650 | 199 | No Matches | . | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| M102 | 1.1 | L20451 | 442 | No Matches | . | AA063651 | 287 | (n) X92098 (p) X92098 Transmembrane protein rp24 (h) | 680 | ||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
| M105 | 1.0 | L20452 | 463 | No Matches | . | AA063652 | 314 | Multiple matchesd | . |
M112 | 1.3 | L20453 | 437 | No matches | . | AA063653 | 328 | (n) AB003102 | Proteasome) subunit (h 1428
| M116 | 1.8 | L20454 | 429 | No Matches | . | . | . | . | . |
M139 | 2.2 | L20455 | 386 | (n) L27843 | Tyrosine Phosphatase (r) f 860 | . | . | . | . |
M140 | 1.3 | L20457 | 406 | (n) L05606 | (p) A46118 muscle myosin binding protein H (h) 809 | AA063654 | 172 | (n) U68267 | (p) L05605 muscle myosin binding protein H (m) 602 | 226 M165 | 1.0 | AA063655 | 403
| (n) U31968 | (p) U31968 muscle-specific Caveolin (n) Z18951 mRNA for caveolin 1313 | 493 685 . | . | . | . |
M177 | 1.9 | L20458 | 373 | (n) M86046 | muscle a-Actinin2 (h) 220 | AA063656 | 321 | (n) M86046 | (p) P35609 muscle a-Actinin2 (h) 1229 | 508 M185 | 1.8 | AA063657 | 394 | (n) X91349 | (p) P45974 De-Ubiquitinase (h) 367 | 180 . | . | . | . |
M186 | 1.8 | AA063658 | 443 | (n) M24088 | Transaminase A (pig) 1153 | . | . | . | . |
M189 | 1.5 | L20459 | 383 | (n) U16368 | muscle a-actin (pi) f 1128 | AA063659 | 308 | (n) U16368 | muscle a-actin (pi) 1388
| M198 | 1.3 | AA063660 | 441 | No matches | . | . | . | . | . |
M218 | 1.7 | AA063661 | 402 | No matches | . | . | . | . | . |
M221 | 1.8 | AA063662 | 472 | (n) Z48042 | (p) S52289 GPI-anchored p137 protein (h) 1104 | . | . | . | . |
M223 | 1.3 | AA063663 | 456 | No matches | . | AA063664 | . | . | No matches
| M239 | 2.3 | AA063665 | 330 | (n) X94252 | muscle desmin (pi) 1121 | AA063666 | 246 | (n) U59167 (h) | (p) P02541 (ha) muscle desmin 945 | 147 M248 | 1.6 | AA063665 | 348 | (n) U07802 | Tis 11d gene (h) 385 | . | . | . | . |
M256 | 0.7 | AA063668 | 420 | No matches | . | . | . | . | . |
M327 | 1.1 | AA063669 | 352 | No matches | . | . | . | . | . |
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Abbreviations after description refers to species of match. h: human;
pi: pig; m: mouse; r: rat; rb: rabbit; y: yeast; ha: hamster.
October 1998
aThe KS and T3/reverse primers initiate DNA sequencing on opposite ends of DNA fragments cloned into pBluescript vectors
(Stratagene), thus two sequences can be produced from each cDNA.
bAccession number of highest scoring, significant database matches is given. (n) nucleotide
level match, (p) protein level match. Score shown was calculated by the blast program (Altschul et al. 1990), cutoff was scores below 200 for blastn and 100 for blastx.
cM29 was sequenced by the universal primer, which sequences the same DNA strand as the KS primer.
dM105 contains a pig-specific repetitive element of the PRE-1 family, thus significant matches were
identified to a number of sequence files. No similarity outside the PRE-1 element was detected.
eLow scoring match of M112 was to a nematode predicted protein coding region of unknown function.
fDuplicate clones for two genes were isolated and sequenced but only one representative clone is listed in the table (M189 for a-actin and M139 for Tyrosine phosphatase).
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